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Report on Results of Chromosome Aberration Test using Cultured Mammalian Cells |
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Report on Results of Chromosome Aberration Test
using Cultured Mammalian Cells
Attached paper 2 3. S9 mix
In
Japanese
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3. S9 mix
(1) Source of S9 (circle the applicable number
and enter requirements.)
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House-made or purchased
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1. House-made 2. Purchase (supplier:
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Date of production (Y/M/D)
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Lot No. (in case of purchase)
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Storage temperature
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(2) Preparation of S9
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Animal used
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Inducing substance
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Species and strain
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Name
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Sex
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Administration method
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Age (in weeks)
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weeks old
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Administration period
and ammount
(g/kg body weight)
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Body weight
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g
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(3) Composition of S9 mix
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Constituents
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Amount in
1 ml S9 mix
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Constituents
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Amount in
1 ml S9 mix
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S9
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ml
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NADP
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μmol
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MgCl2
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μmol
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Na-phosphate buffer
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μmol
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KCl
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μmol
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Others (
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μmol
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Glucose 6-phosphate
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μmol
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(4) S9 mix treatment conditions
(circle the applicable number and enter requirements.)
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1. Plate method 2. Suspension
method 3. Others (
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Amount of S9
(final concentration)
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%
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Amount of S9 protein
(final concentration)
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mg/ml
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Treatment time
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h
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Recovery time
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h
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Remarks
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