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JICOSH Home > Guidelines > Report on Results of Chromosome Aberration Test using Cultured Mammalian Cells
Guideline

Report on Results of Chromosome Aberration Test
using Cultured Mammalian Cells

Attached paper 2    Appendix 1
In Japanese

Appendix 1  Chromosome aberration test results (short-term treatment process)

Name of test substance

Treatment
time (h)

S9
mix

Dose of
test
substance
(mg/ml)

Number of cells showing structural chromosome aberration (incidence, %)

Number of
gap
appearances

Cell
growth
index (%)

Number of cells showing numerical chromosome aberration
(incidence,  %)

Number of cells observed

Chromatid break

 Chromatid exchange

Chromosome
break

Chromosome exchange

Others

Total number of aberration cells (%)

Number of cells observed

Polyploid

Others

Total number of aberration cells (%)

–

–

Negative control
(     )































(      )




(      )

–

–
































(      )




(      )

–

–
































(      )




(      )

–

–
































(      )




(      )

–

–
































(      )




(      )

–

–

Positive control
(     )































(      )




(      )

–

+

Negative control
(     )































(      )




(      )

–

+
































(      )




(      )

–

+
































(      )




(      )

–

+
































(      )




(      )

–

+
































(      )




(      )

–

+

Positive control
(     )































(      )




(      )

[Remarks]
1.

Enter in order of treatment time and recovery time in the column of treatment time.
2. Enter in order of lower dose for the dose of test substance.
3. Enter solvent and negative control substance in the parentheses.  Enter full name of the substance name in the margin when abbreviations are used in the table.
4. Enter the data in accord with the plate of each group in first and second lines and enter the total in the third line.
5. Place the mark † next to the dose if precipitation of the test substance is observed.
6. Enter TOX in the column of the number of cells observed when the dose at which the chromosome observation was disabled due to cell growth inhibition. is stated.e column of "others" is used, state the contents in the margin.
7. When the column of "others" is used, state the contents in the margin.


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