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Report on Results of Chromosome Aberration Test using Cultured Mammalian Cells |
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Report on Results of Chromosome Aberration Test
using Cultured Mammalian Cells
| Attached paper 2 |
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5. Test by short-term treatment process |
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(1) |
Conditions of cell growth inhibition test |
In
Japanese
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5. Test by short-term treatment process
(1) Conditions of cell growth inhibition test
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Case not by metabolic activation method
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Case by metabolic activation method
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Test period
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m d
y to
m d y
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m d
y to
m d y
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Incubator
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Form
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Size
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Amount of culture medium
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ml/incubator
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ml/incubator
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Number of incubators per dose
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Cell
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No. of cells seeded
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pcs./ml
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pcs./ml
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Period of pre-culture
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days
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days
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Treatment conditions
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Amount of test substance solution
added
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ml/incubator
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ml/incubator
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Amount of S9 mix added
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ml/incubator
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Final concentration of S9
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Final concentration of S9
protein
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Treatment time
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h
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h
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Recovery time
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h
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h
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Method for measurement of
cell growth inhibition
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Remarks
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