JICOSH was closed in 2008. For further information, please contact JISHA.
JICOSH Home >
Guidelines >
Report on Results of Chromosome Aberration Test using Cultured Mammalian Cells |
 |
 |
|
Report on Results of Chromosome Aberration Test
using Cultured Mammalian Cells
Attached paper 2 |
|
5. Test by short-term treatment process |
|
|
|
(3) |
Conditions of Chromosomal Aberration Test |
In
Japanese
|
|
(3) Conditions of
Chromosomal Aberration Test
|
Case not by metabolic activation method
|
Case by metabolic activation method
|
Test period
|
m d
y to
m d y
|
m d
y to
m d y
|
Incubator
|
Form
|
|
|
Size
|
|
|
Amount of culture medium
|
ml/incubator
|
ml/incubator
|
No. of incubators for each
dose
|
|
|
Cell
|
No. of cells seeded
|
pcs./ml
|
pcs./mL
|
Period of pre-culture
|
days
|
days
|
Treatment conditions
|
Amount of test substance solution
added
|
ml/incubator
|
ml/incubator
|
Amount of S9 mix added
|
|
ml/incubator
|
Final concentration of S9
|
|
|
Final concentration of S9
protein
|
|
|
Treatment time
|
h
|
h
|
Recovery time
|
h
|
h
|
Remarks
|
(4) Chromosomal Aberration Test Results (by Appendix-1)
|
|
|