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JICOSH Home > Guidelines > Report on Results of Chromosome Aberration Test using Cultured Mammalian Cells
Guideline

Report on Results of Chromosome Aberration Test
using Cultured Mammalian Cells

Attached paper 2   5. Test by short-term treatment process
(3)   Conditions of Chromosomal Aberration Test

In Japanese


(3)          Conditions of Chromosomal Aberration Test

  

Case not by metabolic activation method

Case by metabolic activation method

Test period

   m     d      y   to        
m     d      y 

   m     d      y   to    
m     d      y 

Incubator

Form

     

Size

     

Amount of culture medium

ml/incubator

ml/incubator

No. of incubators for each dose

     

Cell

No. of cells seeded

pcs./ml

pcs./mL

Period of pre-culture

days

days

Treatment conditions

Amount of test substance solution added

ml/incubator

ml/incubator

Amount of S9 mix added

  

ml/incubator

Final concentration of S9

     

Final concentration of S9 protein

     

Treatment time

h

h

Recovery time

h

h

Remarks




(4)          Chromosomal Aberration Test Results (by Appendix-1)




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